Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell
- Sushma Kalmodia1, 2,
- Suryanarayanan Vandhana1,
- B. R. Tejaswini Rama2,
- Balasubramanyam Jayashree†1,
- T. Sreenivasan Seethalakshmi†1,
- Vetrivel Umashankar3,
- Wenrong Yang2,
- Colin J. Barrow2,
- Subramanian Krishnakumar†1Email author and
- Sailaja V. Elchuri†1Email author
© Kalmodia et al. 2016
Received: 30 October 2015
Accepted: 5 January 2016
Published: 9 February 2016
Functionalized gold nanoparticles are emerging as a promising nanocarrier for target specific delivery of the therapeutic molecules in a cancer cell, as a result it targeted selectively to the cancer cell and minimized the off-target effect. The functionalized nanomaterial (bio conjugate) brings novel functional properties, for example, the high payload of anticancer, antioxidant molecules and selective targeting of the cancer molecular markers. The current study reported the synthesis of multifunctional bioconjugate (GNPs-Pep-A) to target the cancer cell.
The GNPs-Pep-A conjugate was prepared by functionalization of GNPs with peptide-A (Pro-His-Cys-Lys-Arg-Met; Pep-A) using thioctic acid as a linker molecule. The GNPs-Pep-A was characterized and functional efficacy was tested using Retinoblastoma (RB) cancer model in vitro.
The GNPs-Pep-A target the reactive oxygen species (ROS) in RB, Y79, cancer cell more effectively, and bring down the ROS up to 70 % relative to control (untreated cells) in vitro. On the other hand, Pep-A and GNPs showed 40 and 9 % reductions in ROS, respectively, compared to control. The effectiveness of bioconjugate indicates the synergistic effect, due to the coexistence of both organic (Pep-A) and inorganic phase (GNPs) in novel GNPs-Pep-A functional material. In addition to this, it modulates the mRNA expression of antioxidant genes glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) by two–threefolds as observed.
The effects of GNPs-Pep-A on ROS reduction and regulation of antioxidant genes confirmed that Vitis vinifera L. polyphenol-coated GNPs synergistically improve the radical scavenging properties and enhanced the apoptosis of cancer cell.
KeywordsAntioxidant peptide Conjugate Cancer cell Gold nanoparticles (GNPs) Oxidative stress Reactive oxygen species (ROS)
Functional nanomaterials are used for various biomedical applications, and it is important to devise a safe and cost-effective alternative approach for targeted cancer therapy. Functionalized gold nanoparticles (GNPs) as an emerging and promising nanocarrier, particularly for the application in nanoparticle-mediated targeted therapy, have shown a significant progress in cancer therapy and diagnosis. Among all nanomaterials, GNPs are more promising due to their unique physicochemical properties, such as biocompatibility, surface plasmon resonance (SPR), and ability to functionalize with amine and thiol groups (Choi et al. 2010; Koshevoy et al. 2011). The therapeutic efficacies of GNPs were demonstrated by various studies which include the multifunctionality and specific interaction of target moieties, such as peptide, antibody, DNA/RNA, and drug with cellular molecular markers (Delong et al. 2010; News and Views in 2010; Thundimadathil 2012). The peptide favors therapeutic application due to its small size, ease of synthesis, tumor-penetrating ability, bioavailability, and good biocompatibility (Agyei and Danquah 2011; Gu et al. 2012; Prades et al. 2012). Peptides such as cell-penetrating peptides, therapeutic peptides, and antioxidant peptides have been reported earlier for cancer therapeutic applications (Thundimadathil 2012; Flora and Pachauri 2010 ; Farkhani et al. 2014). The antioxidant molecules increase hydrogen peroxide levels, scavenge reactive oxygen species (ROS), and induce cancer cells to undergo apoptosis (Turkevich 1951). Thiol-based antioxidant systems are present in mammals to provide antioxidant property and, therefore, antioxidant molecules are important molecules for disease prevention (Valko et al. 2006; Zeng et al. 2015).
The redox imbalance in a cancer cell is correlated with the oncogenic stimulation and important indicator of the disease progression. Therefore, it has considered as a pro-tumorigenic signaling molecule that, involved in the initiation, progression and metastasis of cancers (Maeda 2009; Valko et al. 2007; Storz 2005; Droge 2002; Finkel 2003; Kimura et al. 2005). Hence, modulating the redox state of the cancer cell is an interesting area of study for cancer therapy. Several studies reported the nanocarrier-based targeting of the cancer biomarkers such as, transforming growth factor-beta-1 (TGF-β1), transmembrane receptor P185(HER2) for targeted cancer therapy, to our knowledge, none of these studies utilized to target the ROS using antioxidant peptide (Pep-A) (Prades et al. 2012; Tsai et al. 2013; Kodiha et al. 2015). Hence, it is imperative to develop a suitable biocompatible functionalized nanomaterial for specific and effective targeting the cancer tissue.
In this study, we used RB cancer model for targeting the ROS, by means of the antioxidant peptides (Pep-A) which contain alternative aromatic or sulfur-containing amino acid. The side chains of Pep-A are believed to contribute to strong radical scavenging activities of peptides in the cancer cell. Toward achieving this aim, we design novel multifunctional strategies to attain more specific and effective response. The application of antioxidant molecule alone for targeted therapy has limited its application due to bioavailability and stability in biological environment, limitations of which prompted this research of utilizing nanocarrier-mediated delivery, as it could play a pivotal role of being an effective therapeutic agent. Hence, GNPs-Pep-A composite was synthesized using thiotic acid (TA), an antioxidant linker molecule. Thiotic acid improves the metal-chelating capacity, radical scavenging, and improves the antioxidant effect. The GNPs synthesized by Vitis vinifera, L is chosen for the delivery of Pep-A due to several advantages associated with the natural polyphenols used in the synthesis processes: (1) antioxidant and anticancer property, (2) reduced chemical and metal toxicities (3) synergistic improvement in the antioxidant effect of Pep-A, and (4) most importantly, decrease in the nanoparticle-mediated ROS induction (Sharma et al. 2011). The current findings indicate that bioconjugate (GNPs-Pep-A) shows therapeutic efficacy by quenching the ROS species in the cancer cells. The antioxidant potency of bioconjugate to target the ROS is owing to the co-existence of different phases in a conjugate (GNPs-Pep-A).
Although a number of nanocarrier-based approaches have emerged for cancer therapy, this study is an aid for ROS-mediated targeted therapy. It is an effective and safe approach for the diseases like cancer which are highly heterogeneous in nature. Thus, the current study provides a rational plan with an alternative to conventional peptide-functionalized targeted therapy, which would be an effective strategy in the clinical management of cancer.
Evaluation of antioxidant effect of peptides A and B (Pep-A and Pep-B)
Two short peptides, Pep-A and Pep-B, were selected and evaluated for antioxidant efficiency by measuring their effects on cellular reactive oxygen species (ROS) and superoxide dismutase (SOD) enzyme activity on cultured Y79 retinoblastoma cells. The antioxidant peptides, Pep-A: Pro-His-Cys-Lys-Arg-Met (PHCKRM) and Pep-B:Thr-Arg-Asn-Tyr-Tyr-Val-Arg-Ala-Val-Leu (TRNYYVRAVL), were procured from AnaSpec, Bi Biotech India Pvt Ltd, New Delhi. The thiotic acid-modified Pep-A peptides were custom synthesized by Custom Peptide Synthesis, USV limited India, CPS 1514-1. The peptides were HPLC purified to obtain >95 % purity.
Y79 retinoblastoma cell line (RCB1645) was obtained from RIKEN BioResource Center cell bank (Ibaraki, Japan) and maintained with an atmosphere containing 5 % CO2 at 37 °C in RPMI 1640 media (Sigma-Aldrich, USA) with 10 % FBS. After it attains 80–90 % confluence, the cells were used for all the in vitro experiments. Muller-glial (MIOM1) cell line derived from the neural retina (Limb et al. 2002) was grown in DMEM media supplemented with 10 % FBS and maintained at 37 °C with 10 % CO2. Muller-glial cell line was a gift from Dr. G. A. Limb, the Institute of Ophthalmology, University College, London.
Measurement of intracellular ROS levels on peptide treatment using 2′, 7′ Dichlorodihydrofluorescein (DCF) assay
The intracellular ROS levels were measured using an OxiSelect ROS Assay Kit (Cell Biolabs Inc, CA, USA) as per the manufacturer’s instructions. Y79 cels, RB cell lines, were seeded at 7 × 103 cells/well in 96-well plates and incubated at 37 °C overnight. Then, the cells were washed thrice with sterile PBS and treated with 100 μl of 1X DCFH-DA (2′, 7′-Dichlorodihydrofluorescin diacetate) for 1 h at 37 °C. The solution was removed, and the cells were washed with sterile PBS and treated with varying concentrations (ranging from 10 to 100 µM) of Pep-A and B for 24 h. The reaction was terminated by adding 100 μl of cell lysis buffer, mixed thoroughly for proper cell lysis, and incubated for 5 min. The fluorescence was read on a Spectra Max M4 multi-detection micro plate reader (Molecular devices, CA, USA) at 480-nm excitation/530-nm emission. The ROS levels in cell lysates were then calculated using the DCF standard curve.
SOD activity measurement
Y79 cells were seeded at 2 × 105 cells/well in 12-well plates with 1000 μl of culture media and incubated at 37 °C overnight. The cells were then exposed to varying dosages of Pep-A and Pep-B (10, 50, and 100 μM) in fresh medium and then incubated for 6 h. At the end of the incubation, the cells were collected and washed with ice-cold PBS twice and lysed with cell lysis buffer [0.1 M Tris/HCl, pH 7.4 containing 0.5 % Triton X-100, 5 mM β-Mercaptoethanol, 0.1 mg/ml PMSF]. Cell lysate was centrifuged at 14000×g for 5 min at 4 °C. The supernatant contains a combined SOD activity from cytosol and mitochondria. The SOD activity was measured using the superoxide dismutase (SOD) activity assay kit, (BioVision, CA, USA, Sigma Aldrich, MO, USA) as per the manufacturer’s instructions. In brief, the cell lysate, buffer, enzyme, and the WST reagent were diluted, and the solution was added as per the protocol and incubated at 37 °C for 20 min and read at 450 nm on a microplate reader (Biotek, VT, USA). SOD activity was then calculated as a percentage of the inhibition activity of xanthine oxidase.
Y79 retinoblastoma and MIOM1 cells were seeded at 5 × 103 cells/well in 96-well plates and incubated at 37 °C overnight. The cells were then treated with varying concentrations (10, 30, 60, and 100 µM) of Pep-A and Pep-B in a fresh medium and incubated for specific time periods (24 and 48 h). At the end of the incubation, 10 µl of MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; 5 mg/ml] was added to the cells with fresh medium (100 µl) and incubated at 37 °C until formazan crystals were formed. The formazan crystals were dissolved in 100 µl of DMSO, and the reading was taken at 570 nm (BioTek, ELISA, USA). Cell viability was calculated as Test OD/Control OD × 100.
Preparation of bioconjugate (GNPs-Pep-A)
The filtered GNPs were used to prepare the bioconjugate (GNPs-Pep-A), and the conjugation protocol was followed as described by N. Chanda et al (Chanda et al. (2010). The thiotic acid (TA)-modified peptide conjugates with GNPs in different ratios of peptides, for example, GNPs:Pep-A1 [(18 ml GNPs: 1.7 mg) and GNPs:Pep-A2 (18 ml GNPs: 4.3 mg)] were used. The conjugates were prepared using 18 ml of GNPs (OD520 nm = 1.00), [2.32 × 10−9, Molar particles/L, (242 × 10−6, Moles of gold/L)] mixed with 4.3 mg of Pep-A dissolved in 1 ml of water and kept under stirring overnight. A similar protocol was followed for the preparation of other conjugate, and the synthesized conjugate was subjected to purification. GNPs-Pep-A was washed twice by centrifugation at 15,000 rpm for 30 min with distilled water to remove unattached peptides on the surface of the gold. The thiotic acid (TA) has sulfur-containing antioxidant linker molecules that bind with GNPs to form Au–S bond between peptide and GNPs (Yin 2007). The GNPs-Pep-A was pelleted, and the supernatant was measured for the presence of peptide by reading the absorption at 280 nm. The 50 µM was the final concentration of GNPs-Pep-A which was used for the in vitro studies. The conjugate (GNPs-Pep-A) concentration was measured using the GNP’s concentration.
In vitro stability of GNPs-Pep-A
The stability of GNPs-Pep-A was studied in a variety of solvents and buffers using previously reported methodology (Kalmodia et al. 2013). One ml of GNPs-Pep-A was mixed with 0.5 ml (10 mM) each of DTT, cysteine, histidine, and NaCl; also, GNPs alone was used as a reference. The solutions were then incubated for 2 h at room temperature, and the stability of the GNPs-Pep-A was determined using UV–Vis spectrophotometer (DU 800 Spectrophotometer, Beckman Coulter Inc, CA, USA).
UV–Vis spectroscopy of GNPs-Pep-A
The GNPs-Pep-A were characterized using UV–Visible spectroscopy (Molecular device-M4, CA, USA) to observe the surface plasmon resonance (SPR) with respect to the GNPs. The spectra were obtained in the range of 400–800 nm using water as a blank.
FT-IR characterization of GNPs-Pep-A
The lyophilized GNPs-Pep-A was subjected to Fourier transform-infrared spectrum (FT–IR) analysis for characterizing the functional groups using an FT–IR Spectrometer (Vortex 70 BRUKER, Billerica, MA 01821, USA). The samples were mixed with KBr powder, pulverized, and formed into a disk-shaped pellet. FTIR spectra were recorded in the frequency range of 500–4000 cm−1 in transmittance mode.
Size and zeta potential measurement by dynamics light scattering (DLS) of GNPs-Pep-A
The average hydrodynamic size (Z average), size distribution (% number distribution), and zeta potential of GNPs-Pep-A as a colloidal suspension were measured using dynamic light scattering (DLS) and a Zetasizer [Nano-ZS model equipped with 4.0 mW, 633 nm laser, Model ZEN3600, serial no. MAL1024433, Malvern Instruments Ltd., Malvern, UK]. The particle size was determined based on the Brownian motion relating to the size of the particles suspended in a liquid.
Uptake and internalization by fluorescent-activated cell sorter (FACS)
The Y79 cells were seeded on the lysine (0.01 mg/ml)-coated glass slide. The cells were cultured overnight and treated with different concentrations (50 or 100 µM) of FITC-tagged Pep-A for 4 h, for studying the localization and internalization of the peptide by fluorescence microscopy. The FITC-labeled peptide A molecules (molecular mass 771.2) were custom synthesized (CPS 1468-1, custom peptide synthesis, USV limited India) with ~95 % purity obtained by HPLC method. The GNPs and GNPs-Pep-A conjugates were doped with the R6G dye as described by Kalmodia et al., for the internalization study (Kalmodia et al. 2013). The internalization of the peptide, R6G-doped GNPs and GNPs-Pep-A after 4 h was qualitatively detected by Zeiss LSM, 710 laser microscope supplied by Axio vision (Germany). The peptide uptake was performed by flow cytometric analysis to confirm the concentration-dependent uptake of the peptide in cell culture in vitro. Y79 cells were seeded at 2 × 105 cells/well in six-well plates and treated with 50 and 100 µM concentrations of Pep-A for 4 h. The cells were then spun at 1500 rpm for 5 min, washed with PBS buffer, and then resuspended in FACS buffer. The cells were then analyzed for uptake of the peptide using FACS Calibur (BD Biosciences, CA, USA) by means of CELL QUEST PRO software. The fluorescence of FITC-stained cells was excited with an argon laser at 488 nm, and the emissions were detected at 530 nm. The overlay graph and scattered plot were used to confirm the FITC positive cell population.
Functional effects of GNPs-Pep-A
Primer sequences for target genes used in qRT-PCR
FP 5′- TCTGGAGAAGTGCGGAGATT-3′
RP 5′- AGTCAGGGTGGACCTCAGTG-3′
Superoxide dismutase 1 (SOD1)
FP 5′- GATGAAGAGAGGCATGTTGGAGAC-3′
RP 5′- GTCTTTGTACTTTCTTCATTTCCACC-3′
Glutathione peroxidase (GPX)
FP 5′- GCACCCTCTCTTCGCCTTC-3′
RP 5′- TCAGGCTCGATGTCAATGGTC-3′
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
FP 5′-GCCAAGGTCATCCATGACAAC -3′
The in vitro data were analyzed by one-way ANOVA using SPSS-17 software, and the post hoc test was performed for multiple comparisons. Student’s unpaired t test was used for statistical comparisons, and P < 0.05 was considered significant.
Results and discussion
In vitro antioxidant activities of peptide A and peptide B
The effects of 10–100 μM of Pep-A concentrations were studied on the SOD enzyme activity. The enzyme activity decreased by 0.5 and 0.7-folds at 10 and 50 µM Pep-A concentrations, respectively (Fig. 2b), and increased by 1.79-folds at 100 µM Pep-A treatment, indicating that this concentration could be ideal for the treatment on Y79 a, RB cells (Fig. 2b). Furthermore, the Pep-A could be involved in decreasing the ROS (Fig. 2a) by increasing the antioxidant enzyme activity. A similar increase in the antioxidative enzyme levels in the presence of Hoki skin antioxidative peptide in hepatocarcinoma cells is attributed to the peptide’s role in maintaining the redox balance in the cellular environment (Jridi et al. 2014). The concentration- and time-dependent effects of inhibition of Lypooxigenase 12 enzyme levels by a peptide were reported in the breast cancer cells. This study suggested that the therapeutic effect of the peptide depends on concentration, cell line, and the duration of treatment (Singh et al. 2012). Pep-B used in the study did not show any increased SOD enzyme activity at different concentrations (Fig. 2b), indicating that this peptide did not elicit any change in the cellular antioxidant enzymes. Therefore, further work is carried out using Pep-A. The hybrid bio-nanoparticle is synthesized using GNPs, 100 µM Pep-A, and thiotic acid as linker molecules. The concentration of the peptide used was 100 µM which was found to be effective to elicit the antioxidant enzyme response by this peptide (Fig. 2b).
Characterization of bioconjugate (GNP-Pep-A)
The Pep-A bind to GNPs, which is evaluated by means of FTIR vibrational spectroscopy as this method gives specific vibrational patterns for the ligand bound to GNPs (Tiwari et al. 2014). Spectroscopic analysis of GNPs-Pep-A by FTIR (Fig. 3c) showed vibrational bands at 3446–2365, 2620, and 1641–663 cm−1. The presence of 1641 and 1669 cm−1 bands in the FTIR spectra of Pep-A and GNPs-Pep-A confirms the presence of the amide bonds. The peptide coating on the GNPs exhibited a characteristic IR band at 3448 cm−1, indicating the presence of O–H and COOH moieties, whereas 2920 and 2854 cm−1 bands can be assigned to the symmetric and anti-symmetric vibration bands for CH2 and CH3 groups and C = O stretching of an amide I. In a previous study, COO− bands assigned at 1600 and 1390 cm−1are asymmetric and symmetric, whereas a band at a broad range 3000–3500 cm−1 range was assigned for NH3 +of amino acid (Khlebtsov 2011). The GNPs-Pep-A nanoparticles showed significant peak shift from 1641 to 1669 cm−1 compared to Pep-A alone indicating the effect of metal surface on selective surface–induced functional group shift. Similar changes in vibrational modes in the presence of metallic nanoparticles have been observed before (Alexander et al. 2002). The shift in the vibration peak intensities may indicate covalent binding of Pep-A to the GNPs to form GNPs-Pep-A (Aryal et al. 2006). The peaks at 2129 and 2365 cm−1 in the spectra of the Pep-A indicate S–H bond in the peptide molecule, whereas the disappearance of these peaks in GNPs-Pep-A may suggest the absence of S–H bonds and the presence of the Au–S bond (Kalmodia et al. 2013).
Stability of the GNPs-Pep-A
Effects of different solvents on stability of GNPs-Pep-A
Zeta potential (mV)
Z Average (hydrodynamic size)
−22.5 ± 1.77
49.6 ± 0.4
Conjugate (GNPs-Peptide A)
−26.5 ± 2.02
91.17 ± 1.17
DTT + conjugate
−35.4 ± 3.95
92.36 ± 0.30
NaCl + conjugate
−22.6 ± 1.98
89.48 ± 0.31
Cysteine + conjugate
91.05 ± 0.99
Histidine + conjugate
−32.0 ± 0.25
95.96 ± 0.88
The stability of the GNPs-Pep-A was tested in different solvents using UV–Visible spectroscopy and DLS measurements (Fig. 3a and b). The absorption peak of GNPs-Pep-A at 540 nm remained unaltered in various solvents except for the solvent-containing histidine which shows increase in absorbance (Fig. 3a) indicating the binding activity to form GNPs-Pep-A hybrid. The histidine has a positive charge on its imidazole functional group which brings about changes in the binding affinity with the metal nanoparticles (Flora and Pachauri 2010). The zeta potential of GNPs-Pep-A-His (32.0 ± 0.25) is higher than GNP-Pep-A(−32.0 ± 0.25) indicating a greater stability in the presence of histidine (Table 2). The sulfur-containing amino acid cysteine and DTT also increased the zeta potential to −36.96 ± 1.17 and −35.4 ± 3.95, respectively (Table 2). The cystine amino acid could increase the acidity of the solution thereby increasing the zeta potential of the nano particle as evident from the increased potential in the presence of DTT and amino acid. In the presence of high ionic strength salt NaCl, the zeta potential does not alter. The conjugated Pep-A to GNPs maintains the electric double layer of the GNPs and decreases the probability of the salt Na+ ions to cause any aggregation to the hybrid (Bei et al. 2011; Liu et al. 2007). Thus, the GNPs-Pep-A is stable in various biologically relevant solutions, making these characteristics suitable for their potential applications.
Internalization and uptakes of Pep-A, GNPs-Pep-A, and GNPs
The optical microscopic images of the Y79 cells, RB cell line, treated with the peptide, GNPs, and GNPs-Pep-A (Fig. 5) show the morphological differences compared to the untreated cells. The cells treated with the peptide alone (Fig. 5 panel-2) show similarity in morphology as the control untreated cells (Fig. 5 panal-1), whereas the cellular morphologies of treated cells with GNPs and GNPs-Pep-A (Fig. 5 panel 3 and 4) showed significant differences. The cell fragments were observed, indicating apoptotic bodies formed during the process of membrane cell death. The clustering of cells, shrinkage, change in size, and condensation of cytoplasm is in accordance with the apoptotic or necrotic cell death and are the most commonly observed microscopic features (Barros et al. 2003). The better performance of the GNPs and GNP-Pep-A compared to the peptide alone in apoptosis of cancer cells indicates the potential application of nanoparticles in cancer therapy.
Antioxidant function of GNPs and GNPs-Pep-A
Further studies were performed to understand the reduction in ROS and its effect on the antioxidant enzyme gene expression. Quantitative real-time PCR analysis with GNPs treatment for the first-line defense antioxidant enzyme gene expression showed twofold downregulations of SOD and GPX, and 2.7-fold downregulation of CAT enzyme mRNA levels (Fig. 6d). GNPs-Pep-A showed 2.1-fold upregulation of the CAT and SOD, and 2.5-fold upregulation of GPX enzyme gene expression (Fig. 6d). The high up-regulated gene expression level of antioxidant enzymes by GNPs-Pep-A compared to GNPs alone signifies that the GNPs-Pep-A could be a stronger antioxidant response regulator. Furthermore, the polyphenols from grapes, thiotic acid as linker molecules are synergistically acting with Pep-A to decrease the oxidative stress. We further tested enzyme activities to confirm the effect of the gene expression. We chose estimation of SOD enzyme activities after the GNP and GNP-Pep-A treatments to study the inhibition activity for GNPs-Pep-A which was 81 % compared to 61 % for the untreated control; GNPs treatment on Y79 RB cells showed 70 % reduction in SOD activity compared to the control (Fig. 6c). The enzyme activities are consistent with the measured SOD gene expression levels. The treatment of GNP-Pep-A resulted in 81 % decrease in the SOD enzyme activities (Fig. 6c) indicating that the compounds present in the nanoparticles are acting as efficient free radical scavengers bringing down the need for SOD enzyme to scavenge them. The enhanced antioxidant capacity of GNPs-Pep-A could be attributed to the presence of polyphenols on the GNPs, TA, and the presence of antioxidant peptide (Khan et al. 2013). The presence of alternating aromatic and cationic amino acids in the peptides could provide effective antioxidant properties and efficient membrane penetration (Szeto 2006). Thus, the amino acids constituent in a peptide determine its effectiveness. The supra-molecular system utilizes monolayer-protected gold nanoparticles (GNPs) so that the antioxidants and gold nanoparticles improve its functionality. This is consistent with the assertion that the organic molecules in organized assemblies having reactivity higher than the sum of the monomers. Au@Trolox, a gold nanoparticle with a Trolox (vitamin E analog)-derivative monolayer, possesses eight times higher radical scavenging activity compared with the Trolox monomer, indicating that the cooperative effect of supra-molecular assemblies also works in the area of free radical scavenging. Similarly, another study using Atm-deficient mice model confirmed that oxidative stress decreased after treatment of Atm-deficient mice with alpha-tocopherol (Erker et al. 2006).
Cytotoxicity assessment of Pep-A and B
The cancer cells are known to have elevated ROS and altered signaling mechanisms compared to normal cells. The elevated ROS in the cancer cells needs more efficient antioxidants to kill the cells. The GNP-Pep-A bioconjugate was a better antioxidant (Fig. 6) interacting with elevated ROS in the cancer cells causing changes in cell morphologies could be due to membrane blebbing, indicative of apoptosis leading to its death (Fig. 5). Antioxidant treatment strategies can increase hydrogen peroxide levels, scavenge reactive oxygen species (ROS), and induce cancer cells to undergo apoptosis (Gorrini et al. 2013; Trachootham et al. 2009). From, the experimental data, we could relate a similar mechanism operating in the death of cancer cells by the introduction of GNPs-Pep-A conjugate.
Functionalized nanomaterials have revolutionized the field of targeted cancer therapy. Cancer biomarkers or targets such as onco-proteins are targeted with the functionalized nanomaterials. This study emphasizes on targeting the ROS in Retinoblastoma (RB) cancer cells, and also on an in vitro model to deliver the antioxidant peptides. The results have demonstrated an effective system for ROS scavenging activity which induces the apoptosis in RB cancer cells. The observed results confirm the synergistic effect of the GNPs-Pep-A. The novel, multifunctionalized GNPs can be alternative candidates for cancer therapeutic strategy —such as those demonstrated in case of current study—the self-therapeutic GNPs (such as V. vinifrea L. GNPs) and antioxidant peptides. Thus, the antioxidant-based therapy may improve response to the therapy, and therefore, in future, these novel GNPs-Pep-A can be promising nano biocomposites for targeting cancer cells. Although the application of antioxidants is controversial in cancer treatment, the effect of antioxidant can minimize the side effect of chemotherapy, which in turn, can reduce the chemical toxicity, and may, therefore, enhance the degree of success during the course of treatment.
SK: Concept and experiment design, data collection, analysis and interpretation, and manuscript writing; SV: Data analysis and interpretation, manuscript writing; TRBR, BJ, SST, VU, WY, and CJB: Manuscript writing and editing; KS and SVE: Concept, provision of experimental materials, and manuscript writing. All authors read and approved the final manuscript.
The project grants from the Department of Science and Technology (DST) [Grant no: NR/NM/NS/83-2010], the Department of Biotechnology (DBT) [Grant no: BT/PR13091/GBD/27/180/2009 BT/PR3580/PID/6/625/2011], and from Deakin University, Australia by way of scholarship to Sushma Kalmodia) (ID 211823217) are gratefully acknowledged.
The authors declare that they have no competing interests.
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- Agyei D, Danquah MK. Industrial-scale manufacturing of pharmaceutical-grade bioactive peptides. Biotechnol Adv. 2011;29(3):272–7.View ArticleGoogle Scholar
- Amand HL, Rydberg HA, Fornander LH, Lincoln P, Norden B, Esbjorner EK. Cell surface binding and uptake of arginine- and lysine-rich penetrating peptides in absence and presence of proteoglycans. Biochim Biophys Acta. 2012;1818(11):2669–78.View ArticleGoogle Scholar
- Aryal S, Remant BKC, Dharmaraj N, Bhattarai N, Kim CH, Kim HY. Spectroscopic identification of S-Au interaction in cysteine capped gold nano-particles. Spectrochimica Acta Part A Mol Biomol Spectrosc. 2006;63(1):160–3.View ArticleGoogle Scholar
- Barros LF, Kanaseki T, Sabirov R, Morishima S, Castro J, Bittner CX, et al. Apoptotic and necrotic blebs in epithelial cells display similar neck diameters but different kinase dependency. Cell Death Differ. 2003;10(6):687–97.View ArticleGoogle Scholar
- Bei F, Hou X, Chang SLY, Simon GP, Li D. Interfacing colloidal graphene oxide sheets with gold nano-particles. Chem Eur J. 2011;17(21):5958–64.View ArticleGoogle Scholar
- Chanda N, Kattumuri V, Shukla R, Zambre A, Katti K, Upendran A, et al. Bombesin functionalized gold nano-particles show in vitro and in vivo cancer receptor specificity. Proc Natl Acad Sci USA. 2010;107(19):8760–5.View ArticleGoogle Scholar
- Chanda N, Kattumuri V, Shukla R, Zambre A, Katti K, Upendran A, et al. Bombesin functionalized gold nano-particles show in vitro and in vivo cancer receptor specificity. Proceedings of the National Academy of Sciences. 2010;107(19):8760-5.
- Chen J, Zheng A, Chen A, Gao Y, He C, Kai X, et al. A functionalized gold nano-particles and rhodamine 6G based fluorescent sensor for high sensitive and selective detection of mercury(II) in environmental water samples. Anal Chim Acta. 2007;599(1):134–42.View ArticleGoogle Scholar
- Choi CH, Alabi CA, Webster P, Davis ME. Mechanism of active targeting in solid tumors with transferrin-containing gold nano-particles. Proc Natl Acad Sci USA. 2010;107(3):1235–40.View ArticleGoogle Scholar
- Delong RK, Reynolds CM, Malcolm Y, Schaeffer A, Severs T, Wanekaya A. Functionalized gold nano-particles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules. Nanotechnol Sci Appl. 2010;3:53–63.View ArticleGoogle Scholar
- Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82(1):47–95.View ArticleGoogle Scholar
- Elias RJ, Kellerby SS, Decker EA. Antioxidant activity of proteins and peptides. Crit Rev Food Sci Nutr. 2008;48(5):430–41.View ArticleGoogle Scholar
- Erker L, Schubert R, Elchuri S, Huang TT, Tarin D, Mueller K, et al. Effect of the reduction of superoxide dismutase 1 and 2 or treatment with alpha-tocopherol on tumorigenesis in atm-deficient mice. Free Radic Biol Med. 2006;41(4):590–600.View ArticleGoogle Scholar
- Farkhani SM, Valizadeh A, Karami H, Mohammadi S, Sohrabi N, Badrzadeh F. Cell penetrating peptides: efficient vectors for delivery of nano-particles, nanocarriers, therapeutic and diagnostic molecules. Peptides. 2014;57:78–94.View ArticleGoogle Scholar
- Finkel T. Oxidant signals and oxidative stress. Curr Opin Cell Biol. 2003;15(2):247–54.View ArticleGoogle Scholar
- Flora SJ, Pachauri V. Chelation in metal intoxication. Int J Environ Res Publ Health. 2010;7(7):2745–88.View ArticleGoogle Scholar
- Gorrini C, Harris IS, Mak TW. Modulation of oxidative stress as an an ticancer strategy. Nat Rev Drug Discov. 2013;12(12):931–47.View ArticleGoogle Scholar
- Gu L, Zhao M, Li W, You L, Wang J, Wang H, et al. Chemical and cellular antioxidant activity of two novel peptides designed based on glutathione structure. Food Chem Toxicol. 2012;50(11):4085–91.View ArticleGoogle Scholar
- Guterstam P, Madani F, Hirose H, Takeuchi T, Futaki S, El Andaloussi S, et al. Elucidating cell-penetrating peptide mechanisms of action for membrane interaction, cellular uptake, and translocation utilizing the hydrophobic counter-anion pyrenebutyrate. Biochim Biophys Acta (BBA) Biomembr. 2009;1788(12):2509–17.View ArticleGoogle Scholar
- Hällbrink M, Oehlke J, Papsdorf G, Bienert M. Uptake of cell-penetrating peptides is dependent on peptide-to-cell ratio rather than on peptide concentration. Biochim Biophys Acta (BBA) Biomembr. 2004;1667(2):222–8.View ArticleGoogle Scholar
- Jridi M, Lassoued I, Nasri R, Ayadi MA, Nasri M, Souissi N. Characterization and potential use of cuttlefish skin gelatin hydrolysates prepared by different microbial proteases. BioMed Res Int. 2014;. doi:10.1155/2014/461728.Google Scholar
- Kalmodia S, Harjwani J, Rajeswari R, Yang W, Barrow CJ, Ramaprabhu S, et al. Synthesis and characterization of surface-enhanced Raman-scattered gold nano-particles. Int J Nanomed. 2013;8:4327–38.View ArticleGoogle Scholar
- Kamnev AA, Dykman LA, Tarantilis PA, Polissiou MG. Spectroimmunochemistry using colloidal gold bio-conjugates. Biosci Rep. 2002;22(5–6):541–7.View ArticleGoogle Scholar
- Khan MA, Chen HC, Wan XX, Tania M, Xu AH, Chen FZ, et al. Regulatory effects of resveratrol on antioxidant enzymes: a mechanism of growth inhibition and apoptosis induction in cancer cells. Mol Cells. 2013;35(3):219–25.View ArticleGoogle Scholar
- Khlebtsov N, Dykman aL. Biodistribution and toxicity of engineered gold nano-particles: a review of in vitro and in vivo studies. Chem Soc Rev. 2011;40:1647–71.View ArticleGoogle Scholar
- Kimura H, Sawada T, Oshima S, Kozawa K, Ishioka T, Kato M. Toxicity and roles of reactive oxygen species. Curr Drug Targets Inflamm Allergy. 2005;4(4):489–95.View ArticleGoogle Scholar
- Kodiha M, Wang YM, Hutter E, Maysinger D, Stochaj U. Off to the organelles—killing cancer cells with targeted gold nano-particles. Theranostics. 2015;5(4):357–70.View ArticleGoogle Scholar
- Kohen RNA. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. 2002;30(6):620–50.View ArticleGoogle Scholar
- Koshevoy IO, Smirnova ES, Haukka M, Laguna A, Chueca JC, Pakkanen TA, et al. Synthesis, structural characterization, photophysical properties and theoretical analysis of gold(I) thiolate-phosphine complexes. Dalton Trans. 2011;40(28):7412–22.View ArticleGoogle Scholar
- Kosuge M, Takeuchi T, Nakase I, Jones AT, Futaki S. Cellular internalization and distribution of arginine-rich peptides as a function of extracellular peptide concentration, serum, and plasma membrane associated proteoglycans. Bio-conj Chem. 2008;19(3):656–64.Google Scholar
- Limb GA, Salt TE, Munro PM, Moss SE, Khaw PT. In vitro characterization of a spontaneously immortalized human Muller cell line (MIO–M1). Invest Ophthalmol Vis Sci. 2002;43(3):864–9.Google Scholar
- Liu Y, Shipton MK, Ryan J, Kaufman ED, Franzen S, Feldheim DL. Synthesis, stability, and cellular internalization of gold nano-particles containing mixed peptide–poly(ethylene glycol) monolayers. Anal Chem. 2007;79(6):2221–9.View ArticleGoogle Scholar
- Maeda H. Controlling oxidative stress: therapeutic and delivery strategies. Adv Drug Deliv Rev. 2009;61(4):285–6.View ArticleGoogle Scholar
- Maus L, Dick O, Bading H, Spatz JP, Fiammengo R. Conjugation of peptides to the passivation shell of gold nano-particles for targeting of cell-surface receptors. ACS Nano. 2010;4(11):6617–28.View ArticleGoogle Scholar
- News & Views in… Nanomedicine. Nanomedicine: nanotechnology, biology, and medicine. 2010;5(5):683-5.
- Oishi M, Tamura A, Nakamura T, Nagasaki Y. A smart nanoprobe based on fluorescence-quenching PEGylated nanogels containing gold nano-particles for monitoring the response to cancer therapy. Adv Funct Mater. 2009;19(6):827–34.View ArticleGoogle Scholar
- Prades R, Guerrero S, Araya E, Molina C, Salas E, Zurita E, et al. Delivery of gold nano-particles to the brain by conjugation with a peptide that recognizes the transferrin receptor. Biomaterials. 2012;33(29):7194–205.View ArticleGoogle Scholar
- Sanchez C, Belleville P, Popall M, Nicole L. Applications of advanced hybrid organic-inorganic nanomaterials: from laboratory to market. Chem Soc Rev. 2011;40(2):696–753. doi:10.1039/C0CS00136H.View ArticleGoogle Scholar
- Sanchez C, Julian B, Belleville P, Popall M. Applications of hybrid organic-inorganic nanocomposites. J Mater Chem. 2005;15(35–36):3559–92. doi:10.1039/B509097K.View ArticleGoogle Scholar
- Sharma V, Anderson D, Dhawan A. Zinc oxide nano-particles induce oxidative stress and genotoxicity in human liver cells (HepG2). J Biomed Nanotechnol. 2011;7(1):98–9.View ArticleGoogle Scholar
- Singh AK, Singh R, Naz F, Chauhan SS, Dinda A, Shukla AA, et al. Structure based design and synthesis of peptide inhibitor of human LOX-12: in vitro and in vivo analysis of a novel therapeutic agent for breast cancer. PLoS One. 2012;7(2):e32521.View ArticleGoogle Scholar
- Storz P. Reactive oxygen species in tumor progression. Front Biosci J Virtual Libr. 2005;10:1881–96.View ArticleGoogle Scholar
- Szeto HH. Cell-permeable, mitochondrial-targeted, peptide antioxidants. AAPS J. 2006;8(2):E277–83.View ArticleGoogle Scholar
- Thundimadathil J. Cancer treatment using peptides: current therapies and future prospects. J Amino Acids. 2012;2012:967347.View ArticleGoogle Scholar
- Tiwari PM, Eroglu E, Bawage SS, Vig K, Miller ME, Pillai S, et al. Enhanced intracellular translocation and biodistribution of gold nano-particles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis virus. Biomaterials. 2014;35(35):9484–94.View ArticleGoogle Scholar
- Trachootham D, Alexandre J, Huang P. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov. 2009;8(7):579–91.View ArticleGoogle Scholar
- Tsai YS, Chen YH, Cheng PC, Tsai HT, Shiau AL, Tzai TS, et al. TGF-beta1 conjugated to gold nano-particles results in protein conformational changes and attenuates the biological function. Small. 2013;9(12):2119–28.View ArticleGoogle Scholar
- Turkevich John, Stevenson PC, Hillier aJ. A study of the nucleation and growth processes in the synthesis of colloidal gold. Discuss Faraday Soc. 1951;11:55–75.View ArticleGoogle Scholar
- Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44–84.View ArticleGoogle Scholar
- Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006;160(1):1–40.View ArticleGoogle Scholar
- Yin H. Quest for better antioxidants: a commentary on “enhanced radical-scavenging activity by antioxidant-functionalized gold nano-particles: a novel inspiration for development of new artificial antioxidant”. Free Radic Biol Med. 2007;43(9):1229–30.View ArticleGoogle Scholar
- Zeng T, Zhang T, Wei W, Li Z, Wu D, Wang L, et al. Compact, programmable, and stable biofunctionalized upconversion nano-particles prepared through peptide-mediated phase transfer for high-sensitive protease sensing and in vivo apoptosis imaging. ACS Appl Mater interface. 2015;7(22):11849–56.View ArticleGoogle Scholar