Dual role of nanoparticles as drug carrier and drug
© Springer-Verlag 2010
Received: 2 November 2010
Accepted: 7 December 2010
Published: 1 January 2011
The conventional chemotherapeutic agents used in the treatment of human malignancies are directed nonspecifically against both malignant and nonmalignant cells, often limiting their efficacy with having serious side effects. Recent development of drug delivery vehicles has opened up the possibility of targeted drug delivery systems with the potential of achieving maximum efficacy with minimal toxicity. The possibility of using a nanomaterial as a combinational drug component is intuitively evident as it would compensate the toxicity level by enhancing drug delivery efficiency. Additionally, cell-specific cytotoxicity (reported earlier by our group) of the nanovehicle itself may potentiate a more effective targeted cell killing. In this paper, we explore the possibility of using gold nanoparticles playing the dual role of an anticancer agent and a carrier of a chemotherapeutic drug. This is demonstrated using vincristine sulfate (VS), salt of an alkaloid often used in the treatment of multiple myeloma (MM), and U266 as a test MM cell line. The drug VS shows the expected G2-M-phase arrest of cells. Notably, bare gold nanoparticle shows arrest of the S phase cells that may be particularly important in case of slow-growing malignancies like MM where most of the cells remain in G1 phase of the cell cycle. The VS conjugated gold retains the activity of both gold nanoparticle and VS leading to a synergistic rise of the apoptotic cell population.
One important aspect in nanomedicine is focused towards nanoparticle/drug combination. A recent study (Murphy et al. 2008) shows that lipid-cholesterol-based nanoparticle that is targeted towards αvβ3 integrin (an internal receptor of a number of viruses) can work in conjugation with the chemotherapy drug doxorubicin. The greatest advantage in the nanoparticle based drug delivery system is that the drug can remain active at a significantly lower dose (Barbu et al. 2009; Zhao et al. 2009).
Like integrin, cell cycle has been an important target for new drug development in cancer therapy (de Carcer et al. 2007; Johansson and Persson 2008; Dickson and Schwartz 2009; Raucher et al. 2009). The reason for this target identification is that the agents perturbing the cell cycle are not disease specific and can be exploited in different classes of malignancies. As cell cycle is a cellular network-based event, the cycle can be targeted through multiple routes. The antimicrotubule drug vincristine sulfate (VS), salt of a naturally occurring alkaloid, disrupts microtubules and mitotic spindle, resulting in cell-cycle mitotic arrest at metaphase and apoptosis of cells (Sanchez et al. 2000). VS is one of the most important drugs used in the treatment of hematological malignancies as a part of various combination chemotherapy regimens. It is highly lipophilic, and therefore attains a several-hundred-fold higher intracellular than extracellular concentration on administration. However, over expression of the multidrug resistance (Huet et al. 1998; Legrand et al. 1999) transporters P-glycoprotein (PGP) or multidrug resistance-associated protein (MRP) can reduce the intracellular accumulation leading to drug resistance (Calatozzolo et al. 2005; Rauch and Pluen 2007; Ma et al. 2009). Like any chemotherapy drug, VS is also not free from side effects, its dose limiting toxic effect being neurotoxicity. Multiple myeloma (MM) is a differentiated clonal B cell tumor usually comprising slowly proliferating plasma cells (Zhao et al. 2010a, b). MM constitutes around 1% of all malignant diseases and 10% of hematological malignancies. MM is a relatively slow-growing malignancy and is therefore difficult to treat (Dvorak 2006). Most myeloma cells are in the G1 phase of the cell cycle and are hence poor targets for chemotherapy because they have ample time to repair any DNA damage caused by many of the chemotherapeutic drugs before they proceed to the next cell division. VS, being a drug, causing metaphase arrest, form an important component of various antimyeloma combination regimens.
Extending our previous example of NP-based drug delivery systems, we can ask whether it is possible to design a novel NP-based delivery systems that would accentuate the activity of a drug, like VS at lower concentration. While lowering of concentration should lead to minimization of the observed side effects, it is also worth exploring whether it is possible to impart higher efficiency in chemotherapy by arresting the cell at an earlier phase of cell cycle. The direct effect of nanoparticle itself on cell cycle would enhance the chemotherapeutic potential of the drug.
Direct cancer cell killing by gold nanoparticles (GNP) was proposed earlier by our group (Patra et al. 2007) is different from the laser-mediated thermolysis (Lapotko et al. 2006) using GNP. Two disadvantages of the latter approach are the relative inaccessibility of the laser to the target site, and thermal injury induced by the incident laser on nontarget cell confined nanosurface. Recently, Rotello’s group has shown that nanosurface toxicity is indeed possible and the toxicity largely depends upon hydrophobicity of the nanosurface (Daniel et al. 2010). Although Rotello’s work was not aimed for any anticancer drug formulation and it was aimed primarily to evaluate the toxicity of gold nanoparticle, we found the report contextual, as in our view the ‘toxicity’ is a contextual theme whose chemical or biochemical imprint can have differential realization depending on the study system. The important message from this and other works done by Rotello and his group (Chompoosor et al. 2010; Daniel et al. 2010; Zhu et al. 2010), is that nanosurface-induced ROS generation and subsequent DNA damage may give the nanosurface a special role as a drug. This finding inspired us to attempt a nanoformulation in which both the activity of the nanosurface and that of the drug can be combined and the drug delivery problem is also addressed.
2 Materials and methodology
2.1 Preparation of gold nanoparticle
The gold nanoparticles were prepared by the standard method as described by Turkevich and Frens with some modifications (Kimling et al. 2006). In brief, an aqueous solution of HAuCl4 (250 μM, 25 mL) was brought to near boiling condition upto 100°C and stirred continuously with a magnetic stirrer. Freshly prepared trisodium citrate solution (600 μM final concentration) was added quickly, resulting in a change in the solution color from pale yellow to deep red. After obtaining a stable deep red color, the solution was then allowed to cool down to room temperature with continuous stirring.
2.2 Preparation of gold nanoparticle VS conjugate
Freshly prepared aqueous solution of VS was prepared at a stock concentration of 10 μg/ml. Aqueous solution of HAuCl4 (250 μM, 25 mL) was heated to 60°C and stirred continuously with magnetic stirrer. Two working solutions of VS namely, GNP-VS1 and GNP-VS2 at final concentrations of 0.5 μg/ml for GNP-VS1 and 1.0 μg/ml were prepared, respectively. They were then added to the warm auric chloride solution with stirring for 10 min. Trisodium citrate solution (600 μM final concentration) was added quickly and the temperature was increased to near boiling without allowing the solution to boil, resulting in a change in solution color from pale yellow to bluish red. After obtaining a stable color, the solution was then allowed to cool down to room temperature with continuous stirring.
2.3 Characterization of the nanoparticles
The hydrodynamic diameter of the GNP and its conjugate with VS were measured using Malvern NanoZS80 (Malvern, UK). The laser Doppler velocitimetry module of the same instrument was used to measure the zeta potential of the nanoparticle. This technique efficiently measures the relative velocity of tiny particles with respect to the fluid. The zeta potential is purely an electro kinetic property of the electrical double layer surrounding the subject but not the surface of the subject itself. The conjugation was further characterized using Plasmon resonance of the GNP and its conjugates with VS using Thermo UV-Visible Spectrophotometer.
2.4 Cell culture
MM cell line U266 was procured from NCCS, Pune, India and was maintained in DMEM (Gibco-BRL, NY) supplemented with 10% heat-inactivated fetal bovine serum (Gibco-BRL, USA), 5 × 10−5 M 2-ME, 10 mM Hepes, 2 mM l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 1 mM sodium pyruvate. The cell line was terated with nanoparticle concentration (125 μM) and by VS at a concentration that equivalent to that in GNP-VS2 conjugates. All experiments on the treated cell line were done after 72 h.
2.5 MTT assay
In vitro cytotoxicity of GNP and its drug conjugate on MM cells were determined by measuring 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) dye absorbance of living cells. In brief, MM cells (5 × 106 cells per assay) were grown in tissuce culture plates (NUNC, USA). After treatment with GNP and GNP-VS for 48 h, 20 μL MTT (Sigma, USA) solution (5 mg/mL in PBS) was added to each plate and were incubated for additional 4 h at 37°C. Following this, the MTT solution was decanted off and the formazan crystals formed in the viable cells were dissolved with 200 μL DMSO. The absorbance was taken at 570 nm keeping the reference at 405 nm to measure the level of response to GNP and GNP-VS.
2.6 DNA fragmentation assay
The DNA fragmentation was measured after extraction of DNA from a constant number of cells (approximately 1 × 106). In brief, treated and control cells were harvested, pelleted at 1,600 rpm for 5 min, washed twice with PBS, and resuspended in 60 μL of lysis buffer (10 mM EDTA, 50 mM Tris–HCl, pH 8.0, 0.5% SDS, 0.5 mg/mL proteinase-K). The solution was mixed and incubated overnight at 37°C followed by addition of 3 μL of 10 mg/mL DNAse-free RNase-A. After digestion of RNA, gel electrophoresis was performed on a 1.5% agarose containing ethidium bromide. The gels were documented by imaging with UV transillumination.
3 Cell-cycle analysis
Cell-cycle assay for the amount of DNA distribution was determined by staining DNA with PI (propidium iodide) (Sigma). In brief, after treatment for 24 h, nearly 1 × 106 cells were washed in PBS and fixed in 70% ethanol and kept for overnight at −20°C. The fixed cells were washed twice with PBS and then subjected to PI (10 μg) and RNase treatment at 37°C for 1 h. Cell-cycle analysis by flow cytometry was done to determine the percentage of cells in different phases of cell cycle using a FACS Calibur (BD Biosciences, USA) instrument.
4 Annexin V staining
The annexin V staining was performed for the determination and quantitation of the percentage of cells undergoing apoptosis for GNP and its drug conjugate (GNP-VS). In brief, after treatment, the cells were washed twice with cold PBS and resuspended in binding buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 2.5 mM CaCl2) at a concentration of 1 × 106 cells/mL. A 100 μL of the suspended cells in binding buffer was transferred to a 5-mL culture tube, and 5 μL of Annexin V-FITC and 10 μL of PI were added. The solution was mixed properly and incubated for 15 min at room temperature in the dark. At the end of incubation, 400 μL of binding buffer was added, and the cells were analyzed immediately by flow cytometry. Flow cytometric analysis was performed with the FACSCalibur using the CellQuest software (BD, USA).
5.1 GNP and its conjugates with VS
5.1.1 UV-VIS spectroscopy
5.1.2 Dynamic light scattering
5.1.3 Fourier transform infrared spectroscopy
5.2 Anticancer activity
5.2.1 MTT assay
In contrast to the cancer cells, PBMC (peripheral blood mononuclear cells) did not show any alteration in percentage survival pattern when challenged with GNP, VS, GNP-VS conjugates (Fig. 4b).
5.2.2 PI uptake
Figure 4 describes survival rate on the basis of the metabolic activity of the cells. The reducing nature in GNP-VS conjugates seems to retard the metabolic rate of the population. This is illustrated by the relative bar heights in Fig. 4. Importantly, there is also an associated loss of plasma membrane integrity upon such conjugation causing enhancement of PI uptake. Reverse is the case for intact plasma membranes, where PI’s are excluded even in case of cell death (see Fig. 5).
5.2.4 Cell cycle
The cell-cycle pattern was affected by GNP, and in a way that was significantly different from cell-cycle shift induced by VS. In case of GNP, there seems to be an arrest at the S phase of the cell cycle. As shown in Fig. 8a, the G2/M-phase cells are increasing in case of treatment with VS. From the histogram described in Fig. 8a, the GNP-VS conjugates show the cumulative response of both the cell-cycle effectors namely GNP and VS. Figure 8b illustrates the different response of the cell cycle using a contour plot, in which the VS-, GNP-, and the GNP-VS-induced population shift are clearly classified. The analysis further reveals that there is an increase in less-than-2n population. This provides evidence for an onset of DNA fragmentation at the late apoptotic phase of cells in presence of VS, GNP, or GNP-VS.
G2/M population is increased in response to VS as expected, implying a mitotic block, a well-known signature of VS. On the other hand, in case of GNP-VS an additional feature, namely, a decrease of the G0/G1 cell population is thus observed.
Conjugation of drug (here VS) with gold nanoparticle is done successfully by a novel synthesis method. After conjugation, the newly postulated drug (GNP-VS) has achieved unique cell killing properties. The results indicate that Gold conjugated vincristine nanoparticle kills cancer cell line by blocking at synthesis phase (S phase) of cell cycle.
The use of GNP as a drug delivery agent is already established. This is, to our knowledge, the first report describing cell-cycle perturbation by gold nanoparticles. The cumulative effect of GNP and VS on cell cycle makes the combination prospective in chemotherapy of slow-growing cells like MM. The dual role of GNP as a drug carrier and a drug affecting the cell cycle is also an important outcome of the work.
Killing cells remains the backbone of chemotherapeutic approaches to human malignancies. Most of the conventional chemotherapeutic agents are directed nonspecifically against both malignant and nonmalignant cells, limiting the efficacy of the drugs vis-à-vis enhancing their toxicity. As such, intense research is going on to develop newer agents targeted specifically against malignant cells. VS is the salt of a naturally occurring nitrogenous base derived from the pink periwinkle plant, Catharanthus roseus, vincristine is an asymmetric dimeric compound that binds to the protein tubulin and, at low concentrations, inhibits microtubule dynamics. At higher concentrations, it disrupts microtubules and mitotic spindle, resulting in cell-cycle mitotic arrest at metaphase. A smart formulation of the drug (VS) that further affects the cell cycle without invoking any appreciable toxicity is likely to be welcome in MM treatment. As VS is primarily eliminated through the liver into the bile and feces, patients with obstructive liver disease are more susceptible to its toxic effects, requiring a dose reduction depending upon the serum bilirubin concentration. Vincristine uniquely spares the kidney and the bone marrow. As a result, they are continued to be used extensively in the treatment of various hematological malignancies, as a component of combination chemotherapy regimens that also includes more myelotoxic agents. As nanoparticles are shown to reduce the required VS dose (see Figs. 4 and 5), the GNP based drug formulation is expected to reduce the toxic side effects associated with conventional chemotherapy. The arrest at S phase cells by GNP may be of potential important to MM therapy. The renal sparing action of VS has helped design chemotherapy regimens, like vincristine-adriamycin-dexamethasone (VAD) that may be used to treat patients of MM with renal insufficiency. The VAD regimen (Barlogie et al. 1984) has been shown to produce rapid rate of response, but the survival duration achieved is no longer than the most commonly used standard treatment combination of melphalan and prednisolone (Alexanian et al. 1990). It has been shown that the activity of the VAD regimen is almost entirely due to high-dose dexamethasone (Alexanian et al. 1992). Probably only mature plasma cells are killed by dexamethasone, whereas the immature, clonogenic myeloma compartment can be rescued from dexamethasone-induced apoptosis by IL-6, an important cytokine secreted by myeloma cells.
The future use of GNP conjugation with VS and perhaps, the other MM drugs, may open up possibilities for newer chemotherapeutic regime in which this dual role of the nanoparticle is exploited, lower drug concentration may be used and higher drug potential may be achieved at a much reduced level of toxicity.
We thank DST, India (grant no. SR/NM/NS-28/2007) for supporting the research.
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